C&EN

Enzyme conjugate synthesizes DNA

In terms of efficiency, the phosphoramidite method used for synthesizing DNA oligonucleotides is hard to top. Each base-adding step achieves a 99.5% yield. But the method still can’t produce sequences ...
Nature

SUMO-1 conjugation to intact DNA topoisomerase I amplifies cleavable complex formation induced by camptothecin

DNA topoisomerase I (Topo1) manages the topological state of DNA. Cleavable complexes, the covalent Topo1–DNA intermediates, become DNA damaged when the catalytic cycles are inhibited by the ...
GEN

Investigators Use Synthetic DNA and Controlled Conjugation to Replace Every TAG Stop Codon in E. Coli

Combining MAGE with CAGE allowed the genome to manipulated as an editable and evolvable template. Scientists have combined a recently developed technique known as multiplex automated genome ...
JSTOR Daily

Site-Specific Cleavage and Joining of Single-Stranded DNA by VirD2 Protein of Agrobacterium tumefaciens Ti Plasmids: Analogy to Bacterial Conjugation

Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 24 (Dec. 15, 1993), pp. 11538-11542 (5 pages) As an early stage of plant transformation by Agrobacterium ...
JSTOR Daily

Conjugation to Gold Nanoparticles Enhances Polyethylenimine's Transfer of Plasmid DNA into Mammalian Cells

Branched polyethylenimine (PEI) chains with an average molecular mass of 2 kDa (PEI2) have been covalently attached to gold nanoparticles (GNPs), and the potency of the resulting PEI2-GNPs conjugates ...